FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16365849
Sequences flagged as poor quality0
Sequence length100-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG103700.082.162123
CCGAGTA108800.080.487532
GAGTAGC244200.077.699974
CTACTCG154650.076.390222
TACTCGG160050.075.852983
GCTACTC365050.074.384161
AGTAGCT405500.074.3723145
GTAGCTG431050.070.570696
GCTGGGA1075750.070.0392159
CCCGAGT135200.065.723861
TCGGGAG202350.064.894056
GGAGGCT871500.064.8405159
AAGTGCT690400.064.67555
AAAGTGC681200.064.114344
TAGCTGG497650.063.7392777
CCAAAGT734550.062.7292182
CTCGGGA199600.062.098765
CCCAAAG800150.061.686261
CTACTTG239300.061.5948452
AGTGCTG717900.060.8750766
Produced by FastQC (version 0.11.5)