FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16365849
Sequences flagged as poor quality0
Sequence length100-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG98950.083.7326663
GAGTAGC241700.079.548874
CCGAGTA107400.079.3873442
CTACTCG146300.078.7870252
TACTCGG153800.077.450633
AGTAGCT398200.077.248585
GCTACTC347250.075.301831
GTAGCTG418000.073.918476
GCTGGGA1049350.072.667719
AAGTGCT680950.066.960775
AAAGTGC679250.066.62184
TAGCTGG485050.066.339317
CCCGAGT129750.066.310321
CCAAAGT728650.065.9394152
GGAGGCT854400.065.359729
CCCAAAG795350.065.009341
TCGGGAG201250.063.8397756
CAAAGTG739100.062.8470343
AGTGCTG715550.062.520596
TTGGGAG620700.062.4623036
Produced by FastQC (version 0.11.5)