FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13269854
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG55450.072.43433
TACTCGG81700.070.373093
CCGAGTA58300.069.4992452
CTACTCG82000.069.309822
GCTACTC201300.064.4172741
GCTGGGA577350.061.9125639
GAGTAGC142500.061.283934
TCGGGAG105500.058.104866
AGTAGCT241600.057.440365
GGAGGCT475400.057.0355839
CCCGAGT75000.056.8151631
GTAGCTG248400.055.3480956
CTCGGGA108000.055.2495965
ACTCGGG106300.054.470924
AAGTGCT416050.054.3130875
CCCAAAG489050.054.129731
AAAGTGC407300.054.1116684
AGTGCTG418050.053.500526
GTGCTGG417950.052.812337
CCAAAGT454150.052.0783842
Produced by FastQC (version 0.11.5)