FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13269854
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG59800.071.247133
TACTCGG86100.070.950843
CTACTCG85400.070.896132
CCGAGTA64600.067.952072
GAGTAGC143950.066.984114
GCTACTC204100.066.408551
GCTGGGA577650.065.228079
AGTAGCT240050.062.389425
TCGGGAG106850.060.669676
GTAGCTG247950.060.2093246
GGAGGCT480000.059.103489
CTCGGGA105400.058.539135
AAAGTGC404700.057.877424
CCCGAGT79050.057.0963061
ACTCGGG107350.057.095984
AAGTGCT417350.057.0349125
CCCAAAG494150.056.5772361
AGTGCTG421150.056.1003656
GTGCTGG419900.055.9113467
TAGCTGG278600.055.17087
Produced by FastQC (version 0.11.5)