FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7165172
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG28450.060.9430433
CCGAGTA31600.059.167872
CTACTCG27850.053.3976632
GAGTAGC69250.052.3848884
TACTCGG28050.052.353313
CCCGAGT39350.051.7968441
AGTAGCT115550.050.5091065
GCTGGGA233650.050.413789
GCTACTC75150.049.2302361
GTAGCTG117700.048.7593426
CGGAGGT39050.047.5116127
TAGCTGG130100.046.0988247
GGAGGCT181000.045.3266689
TCGCGTT2200.044.8689355
TTGGGAG164400.042.7827266
AAAGTGC153050.042.124184
CCCAAAG192000.041.304551
CTCGGGA35650.041.1875425
AAGTGCT161150.041.0022435
TCGGGAG38500.040.668456
Produced by FastQC (version 0.11.5)