FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7165172
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN85080.11874104348088224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG24300.061.438623
TACTCGG31400.060.1207163
CTACTCG31350.058.8395082
CCGAGTA26700.056.8406752
GAGTAGC68950.053.236814
GCTGGGA235950.052.0284049
CCCGAGT34550.051.9795341
GCTACTC75100.050.949411
AGTAGCT110950.050.0417375
GGAGGCT184100.048.797219
CTCGGGA38150.048.511415
GTAGCTG114050.048.3407676
TCGGGAG41250.048.1396836
TAGCTGG125150.046.1640667
CGGAGGT42750.046.1539847
TTGGGAG168700.046.06036
GTGCTGG154750.044.426247
AGTGCTG154600.044.3973436
CCCAAAG189850.044.0469741
AAAGTGC152550.043.7566834
Produced by FastQC (version 0.11.5)