FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3152430
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACG450.001014526541.7752536
CCGAGTA11500.033.5930862
ACGCGAT851.7290131E-533.2509463
CGAGTAG11250.030.9849593
CTCGCGT1401.8522769E-830.2821164
GCTGGGA68900.029.7356119
CGGAGGT9700.029.5506957
GAGTAGC27650.028.621074
AGTAGCT41100.028.3091555
CCCGAGT16200.027.9241451
CACGCGA1057.306026E-526.9212882
TCGCGTT1057.313145E-526.9174355
TTGGGAG57400.026.855526
TACTCGG9850.026.78083
GTAGCTG43300.026.5919276
TAGCTGG46750.026.435277
GGAGGCT53400.025.3431649
CTACTCG9100.024.850422
GGGAGGC68900.024.7569438
CGCGATG1151.3571387E-424.576794
Produced by FastQC (version 0.11.5)