FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3152430
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN88910.2820363973188937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10950.032.6368752
CGAGTAG10700.032.518933
GCTGGGA69100.029.7618719
AGTAGCT36850.029.4723115
TTGGGAG55550.029.4298526
TACTCGG10250.029.3592113
CTACTCG9950.029.3006842
GAGTAGC23600.029.2877964
AGGCGGA16900.028.9352994
GGAGGCT58900.028.6979839
CGGAGGT13550.028.071487
CCCGAGT15350.027.8981481
GGCGGAG17000.027.6560925
GCTACTC24850.027.4589541
GTAGCTG38800.027.1212186
GGGAGGC72950.025.3595778
GCGGAGG15500.025.1558676
TAGCTGG42500.024.9711847
GCACTTT51600.024.2590771
CCCAAAG52250.024.2274861
Produced by FastQC (version 0.11.5)