FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2853327
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN79970.28026931368188784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1050.049.246645
AGTAGCT49350.044.9601825
TACTCGG11450.044.75173
GTAGCTG51050.042.5106356
CTACTCG12300.042.4242362
GCTGGGA95550.041.2235459
GAGTAGC34500.039.6510544
TTGGGAG75750.039.5630236
TAGCTGG57450.038.74247
CCGAGTA13700.038.432062
CGAGTAG13600.038.36833
GCTACTC28100.038.00811
GCACTTT63300.037.461351
AAAGTGC47500.036.914424
GGAGGCT75100.036.8829889
CCCAAAG58700.036.870271
CTCGGGA14750.036.0130235
AAGTGCT50150.035.994285
CCAAAGT53650.035.926122
CGGAGGT14600.035.378397
Produced by FastQC (version 0.11.5)