FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9548467
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG40550.064.066623
CCGAGTA42500.062.3339422
TACTCGG43400.060.003373
GAGTAGC105300.057.1119774
AGTAGCT172000.056.1682855
CTACTCG46000.055.9612052
GCTGGGA355350.055.324129
CCCGAGT53650.055.144671
GTAGCTG174150.054.6859366
GCTACTC115900.051.91631
TAGCTGG194400.050.7565847
GGAGGCT279000.050.5647859
TCGGGAG57850.050.1971326
CGGAGGT63750.049.274187
CTCGGGA54950.048.754975
TTGGGAG244300.046.1917046
AAAGTGC233050.045.4470864
GTGCTGG231450.045.248847
AAGTGCT235600.044.8491675
ACTCGGG58800.043.9695784
Produced by FastQC (version 0.11.5)