FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8284398
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG32800.063.9640853
CCGAGTA34850.062.3831752
TACTCGG37800.060.789253
GAGTAGC86950.055.6548354
CTACTCG38950.055.495742
AGTAGCT145750.053.9799845
GTAGCTG144850.053.8411986
CCCGAGT46750.053.2477871
GCTGGGA303900.051.2852559
GCTACTC97800.049.4996531
TAGCTGG164250.049.1925857
GGAGGCT229700.048.2520759
TTGGGAG210350.046.6045076
CGGAGGT50600.046.028747
TCGGGAG51400.044.8264166
CTCGGGA48600.044.3254975
TCGCGTT3650.042.76795
ACTCGGG52100.042.546264
AAAGTGC189250.042.3312954
GTGCTGG189650.041.2872057
Produced by FastQC (version 0.11.5)