FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8284398
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG41650.065.207593
CCGAGTA36400.064.66382
CGAGTAG36650.062.6899263
CTACTCG41100.062.4340132
GAGTAGC89550.059.680454
AGTAGCT145450.058.077515
GCTGGGA309550.056.5960359
GCTACTC102050.056.1103741
GTAGCTG148750.056.075536
CCCGAGT48150.054.0787431
GGAGGCT243600.051.2347569
TAGCTGG167850.050.8104747
CTCGGGA52500.049.709675
CGGAGGT56450.049.549357
TTGGGAG207150.049.1886
TCGGGAG56000.048.832626
ACTCGGG55100.048.3838274
AAAGTGC203700.046.5882574
AAGTGCT207700.045.6609765
CCCAAAG244000.045.2459071
Produced by FastQC (version 0.11.5)