FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10336398
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG67100.083.040733
CCGAGTA69800.080.2402952
CTACTCG96750.077.831792
TACTCGG98250.077.502953
GAGTAGC164500.077.3927464
AGTAGCT264800.076.7834855
GCTACTC229700.075.321111
GTAGCTG278250.073.323576
GCTGGGA659250.072.761439
GGAGGCT536800.068.092059
CCCGAGT85750.067.828381
TCGGGAG123150.066.6595466
TAGCTGG319550.066.549037
TTGGGAG412350.064.855736
CTCGGGA121950.064.6366355
AAGTGCT427050.064.484965
ACTCGGG120600.063.5276454
AAAGTGC427600.063.243034
CTACTTG150300.063.206092
CCAAAGT452650.063.1837432
Produced by FastQC (version 0.11.5)