FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10336398
Sequences flagged as poor quality0
Sequence length100-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG64850.084.472373
TACTCGG101350.082.595733
CCGAGTA67650.081.273072
CTACTCG101100.080.415332
GAGTAGC158750.078.505824
GCTACTC238700.078.4323651
AGTAGCT253750.076.790195
GCTGGGA644950.074.891619
GTAGCTG268150.072.55856
AAGTGCT417850.070.542115
GGAGGCT550150.070.19849
AAAGTGC416100.069.865264
TTGGGAG404300.069.072786
TCGGGAG130950.068.972666
CCAAAGT442700.068.4510962
CCCAAAG485100.067.383641
TAGCTGG299800.067.222367
CTCGGGA127000.067.171425
ACTCGGG124100.066.96834
CCCGAGT84000.066.345271
Produced by FastQC (version 0.11.5)