FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8834517
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG55050.080.460033
CCGAGTA57700.078.830782
TACTCGG76150.075.846143
GAGTAGC140500.075.827724
CTACTCG74500.075.291422
AGTAGCT220500.074.126645
GCTACTC173100.073.225681
GTAGCTG232450.069.672316
GCTGGGA530050.068.938649
TCGGGAG95450.064.053426
TAGCTGG261250.063.8838047
AAGTGCT327450.063.7007265
CCCGAGT74050.063.2443121
AAAGTGC325750.063.034834
GGAGGCT430200.062.2153059
CTCGGGA94450.061.4950145
CCAAAGT354150.060.653062
AGTGCTG340350.060.5028156
TTGGGAG331350.060.3991666
ACTCGGG94300.059.9374854
Produced by FastQC (version 0.11.5)