FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7761877
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG32550.056.2003143
CCGAGTA34050.055.944762
GAGTAGC80600.054.193164
TACTCGG31700.053.3890383
GCTGGGA266250.051.600929
AGTAGCT136550.050.71615
CTACTCG32850.049.8397032
GTAGCTG137350.049.6505786
CCCGAGT45450.048.9590261
GCTACTC85900.048.8337821
GGAGGCT209750.047.731179
TAGCTGG152600.046.8908967
TTGGGAG193050.046.2191286
CGGAGGT44650.043.8969467
TCGGGAG42450.042.6537976
TCGCGTT2650.042.2638475
CCCAAAG218450.040.6023561
GTGCTGG172700.040.5326847
AAGTGCT177550.040.161175
AAAGTGC175750.040.041444
Produced by FastQC (version 0.11.5)