FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7761877
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG30600.059.7753943
TACTCGG38050.059.5173073
CCGAGTA33750.058.8041342
CTACTCG37850.055.8861242
GCTGGGA273750.054.6137289
GAGTAGC80700.053.9657554
AGTAGCT133950.053.1813475
CCCGAGT43900.051.7325741
GTAGCTG138850.051.260116
GCTACTC92300.050.3563961
GGAGGCT228000.049.445369
TAGCTGG150800.048.641737
CTCGGGA47350.047.9589235
TTGGGAG204500.046.9735456
TCGGGAG50700.046.8764576
CGGAGGT47650.046.351317
AAAGTGC179100.044.9850234
AAGTGCT180800.044.9408535
GTGCTGG181200.044.395297
AGTGCTG181700.044.1706856
Produced by FastQC (version 0.11.5)