FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8776220
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG50200.078.657293
TACTCGG72750.074.210743
GAGTAGC127650.073.678334
CCGAGTA53950.073.1940842
CTACTCG70700.073.103352
GCTACTC170550.071.859451
AGTAGCT210150.070.7206655
GCTGGGA508900.070.636319
GTAGCTG216750.068.0808646
AAGTGCT326800.066.289825
AAAGTGC321200.065.8872454
GGAGGCT407050.065.0717859
TCGGGAG93150.064.3568346
CCCGAGT63800.063.824621
CCAAAGT356100.063.054092
TTGGGAG309850.062.9964876
TAGCTGG245950.062.752637
CCCAAAG390500.062.5492021
CTCGGGA91500.062.5212755
AGTGCTG342050.062.1506276
Produced by FastQC (version 0.11.5)