FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8776220
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG52800.079.1468663
TACTCGG79500.078.765293
CTACTCG77550.077.601892
GCTACTC180150.076.568841
CCGAGTA56150.075.48222
GAGTAGC126500.073.2081154
GCTGGGA495450.071.869189
AGTAGCT200000.071.8117755
AAAGTGC312550.068.6232764
GTAGCTG208800.068.3296366
TCGGGAG98800.068.033846
AAGTGCT320950.067.771185
GGAGGCT432200.067.339499
CCAAAGT340350.065.847082
CTCGGGA95750.065.740515
CCCGAGT66050.065.287441
AGTGCTG329200.065.178796
CCCAAAG374550.064.853661
TTGGGAG329650.064.490096
ACTCGGG96100.064.2672354
Produced by FastQC (version 0.11.5)