FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8034615
Sequences flagged as poor quality0
Sequence length100-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG53900.085.911463
CCGAGTA56400.082.839432
AGTAGCT219200.078.4015
GAGTAGC135450.077.522614
CTACTCG77250.076.952572
TACTCGG79250.076.5359043
GTAGCTG229150.075.110576
GCTACTC176800.074.9536061
GCTGGGA535100.072.241529
CCCGAGT69900.068.862551
TAGCTGG262900.067.5768667
AAGTGCT322150.067.052315
AAAGTGC323850.066.055884
GGAGGCT427050.065.411839
TCGGGAG99950.065.1874856
CCAAAGT352150.064.465882
CTCGGGA95700.064.0615545
CCCAAAG387500.063.070291
TTGGGAG318550.062.9986766
ACTCGGG95600.062.968794
Produced by FastQC (version 0.11.5)