FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8034615
Sequences flagged as poor quality0
Sequence length100-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG56100.085.5598453
CCGAGTA59750.081.3149952
GAGTAGC136050.081.250524
AGTAGCT214800.080.8517155
TACTCGG79500.079.900013
CTACTCG78650.079.7682
GTAGCTG226650.076.3655556
GCTACTC174000.075.795551
GCTGGGA535400.074.240749
TAGCTGG256300.070.481987
AAAGTGC315350.069.259184
CCCGAGT71650.069.184561
AAGTGCT322100.069.001925
CCAAAGT342450.067.472422
TTGGGAG328050.066.716656
GGAGGCT430150.066.3444069
TCGGGAG102550.065.947066
CCCAAAG377600.065.9155961
CAAAGTG341200.065.693973
AGTGCTG338950.064.455726
Produced by FastQC (version 0.11.5)