FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3206047
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9150.031.8457533
CTACTCG8250.031.3321132
AGTAGCT41250.030.7624385
GGAGGCT53200.030.6557759
GCTGGGA72500.030.4687279
GTAGCTG41150.030.1519246
CGAGTAG11400.029.6830523
GAGTAGC27450.029.2775464
CCGAGTA12400.029.1843472
GCTACTC21000.029.1509341
TTGGGAG57350.028.6824026
TAGCTGG45550.027.961597
CCCGAGT15950.027.4568461
CGGAGGT9450.026.8560967
GGGAGGC71900.025.9503088
CTCGGGA12150.025.5298715
GCACTTT51400.024.7359771
TGGGAGG74850.024.3624447
TCGGGAG12550.023.5927056
CGCGAGT1602.0070456E-623.5449831
Produced by FastQC (version 0.11.5)