FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8781022
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG43150.067.868413
CCGAGTA45300.066.6719362
TACTCGG52200.064.134173
GAGTAGC105850.063.4993824
GCTGGGA394350.061.4869739
AGTAGCT177750.061.148095
CTACTCG53200.061.017132
GCTACTC128450.059.6477471
GTAGCTG182650.059.542976
CCCGAGT59850.056.236341
GGAGGCT312750.054.6564149
TAGCTGG204300.054.4329037
AAAGTGC254800.053.6190574
CCCAAAG308450.053.051041
CCAAAGT285000.052.307332
AAGTGCT264750.052.237395
TCGGGAG68900.051.959676
CTCGGGA66750.051.3139955
AGTGCTG263800.050.8850176
GTGCTGG267950.050.746897
Produced by FastQC (version 0.11.5)