FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9014923
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG60200.075.5033653
CGAGTAG42100.074.805053
CTACTCG61150.072.631082
CCGAGTA44650.072.342712
GAGTAGC107600.065.360814
GCTACTC148650.063.9815981
GCTGGGA404650.063.3406569
CCCGAGT56200.063.0936431
AGTAGCT172850.062.3516775
TCGGGAG77550.061.4962586
GGAGGCT340250.061.0799189
GTAGCTG178450.060.825726
CTCGGGA76550.059.9805835
ACTCGGG78350.058.5181584
TTGGGAG266850.057.3080836
AAAGTGC266350.056.21194
AAGTGCT277800.055.3716055
TAGCTGG205050.054.4835177
CCCAAAG328350.054.4796031
GTGCTGG277250.053.774657
Produced by FastQC (version 0.11.5)