FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11257176
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG47400.069.241663
CCGAGTA48900.068.08422
TACTCGG64150.067.9099043
CTACTCG63650.066.515922
GAGTAGC123900.062.9187284
GCTACTC158550.061.4616281
AGTAGCT207250.060.531095
GCTGGGA465000.060.197419
GTAGCTG212550.059.0389146
TCGGGAG79500.058.079916
GGAGGCT370050.056.2103549
CCCGAGT65800.054.5513271
CTCGGGA80350.054.3806275
TAGCTGG245050.052.6248787
ACTCGGG84050.051.908774
AAGTGCT317950.050.4172825
AAAGTGC313800.050.3325044
TTGGGAG310150.050.300696
GTGCTGG316650.050.0743567
AGTGCTG319100.049.8750576
Produced by FastQC (version 0.11.5)