FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11257176
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG65350.072.671093
CGAGTAG47800.069.4783253
CTACTCG68200.069.4421542
CCGAGTA51600.066.392842
GCTACTC167500.064.186271
GAGTAGC127050.063.3118864
GCTGGGA467950.061.381869
GGAGGCT392000.060.560979
AGTAGCT211300.059.730425
GTAGCTG210350.059.55586
CCCGAGT64100.059.2813871
CTCGGGA82100.059.033965
TCGGGAG86500.058.9756746
TTGGGAG322100.055.9511876
TAGCTGG238150.053.95257
ACTCGGG88200.053.8432164
AAAGTGC317800.053.6514934
AAGTGCT320400.053.5986065
CCCAAAG379500.051.964061
AGTGCTG325000.051.724246
Produced by FastQC (version 0.11.5)