FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10404020
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG81950.076.854963
CGAGTAG56850.075.432163
CTACTCG82400.075.124222
CCGAGTA60300.073.869812
AGTAGCT235550.071.710435
GCTACTC195950.071.706691
GAGTAGC148400.071.073454
GCTGGGA571600.069.6980069
GTAGCTG244300.068.525626
TCGGGAG104250.064.81116
GGAGGCT475200.063.2532469
TAGCTGG283100.062.255967
AAGTGCT368500.062.023765
CCCGAGT75850.061.892811
CTCGGGA104750.061.8766675
TTGGGAG369600.061.591176
ACTCGGG102400.060.8031774
AAAGTGC371150.060.6636434
CCAAAGT395850.060.168422
AGTGCTG381850.059.3409586
Produced by FastQC (version 0.11.5)