FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10404020
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG56400.077.768373
CTACTCG88600.077.656222
TACTCGG89100.077.587813
CCGAGTA59950.073.381632
GAGTAGC145700.072.743214
AGTAGCT234450.072.1239245
GCTGGGA566700.071.795859
GTAGCTG244200.068.628376
AAGTGCT361200.067.911165
GGAGGCT492700.066.714339
AAAGTGC364650.066.5146264
TTGGGAG385250.065.9833156
CCAAAGT394300.065.414912
TCGGGAG112750.064.9134456
CCCGAGT72350.064.340781
AGTGCTG377050.063.806926
CCCAAAG437850.063.7747841
ACTCGGG108200.063.64934
CTCGGGA110500.063.094655
GTGCTGG383000.062.7646837
Produced by FastQC (version 0.11.5)