FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889211
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT42050.037.0744635
CGAGTAG11350.036.9323583
CCGAGTA11800.036.7277262
GTAGCTG43000.036.3986786
GAGTAGC27350.036.3360754
TTGGGAG63450.035.5565386
GCTGGGA79100.034.9911739
TCGCGTT702.1589365E-433.6422355
TAGCTGG48600.032.5846677
GCTACTC21700.032.353421
GCACTTT55200.032.1807371
CCCGAGT14950.031.8327311
GGAGGCT61150.030.8153829
TACTCGG7750.030.3865343
CCCAAAG56550.030.2459851
CTACTCG7850.030.0046522
CACTTTG59100.029.9701252
ACTTTGG57500.029.7338873
TTTGGGA66500.029.1093855
AAGTGCT43700.028.992375
Produced by FastQC (version 0.11.5)