FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882188
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG450.001013276941.78564
CGAGTAG11400.041.6481483
CCGAGTA12300.040.897382
CCCGAGT13750.038.0380331
AGTAGCT45100.037.4194225
GAGTAGC27850.036.9656224
GTAGCTG44500.036.6562966
TTGGGAG64700.036.400946
GCTGGGA81250.035.5715529
TAGCTGG49200.033.7255177
AAAGTGC39600.031.9327454
GGAGGCT58600.031.4368849
GCACTTT55100.031.1277351
AAGTGCT40750.030.6854955
CGGAGGT12000.030.5535967
GGGAGGC80000.030.4879638
CCCAAAG53900.030.3346141
CCAAAGT46600.030.0640282
ACTTTGG58000.029.8262733
TTTGGGA65750.029.8139465
Produced by FastQC (version 0.11.5)