FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9057285
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG32900.061.2021523
CCGAGTA36700.059.8963432
TACTCGG41050.057.843373
CTACTCG40250.056.344832
CCCGAGT49800.054.1202351
GCTACTC100700.053.4031031
GAGTAGC90800.052.5800484
GCTGGGA310300.052.096599
AGTAGCT149000.050.825355
GTAGCTG151900.049.2297486
GGAGGCT247200.048.2071469
CGGAGGT53250.046.969447
TCGGGAG53750.046.414456
TAGCTGG167050.046.3193667
TCGCGTT2750.046.050335
CTCGGGA51550.045.9386675
TTGGGAG223900.044.0604446
ACTCGGG56700.042.4361534
GTGCTGG207600.041.9080357
AAAGTGC211450.041.713724
Produced by FastQC (version 0.11.5)