FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9057285
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA37500.061.6258132
TACTCGG44150.061.52163
CGAGTAG34950.061.4120453
CTACTCG45000.058.1083532
GCTACTC106350.055.4974441
GAGTAGC92650.055.2848974
CCCGAGT48900.053.4854621
GCTGGGA311050.053.1669279
CTCGGGA53100.052.106315
AGTAGCT151850.051.618965
GTAGCTG150100.051.4190446
GGAGGCT251800.051.0684669
TCGGGAG57700.050.9147036
CGGAGGT57650.047.883497
TTGGGAG223450.047.8575366
TAGCTGG168100.047.6831137
ACTCGGG60050.045.3376354
AAAGTGC209650.043.0955624
AAGTGCT212450.042.7361955
GGGAGGC316100.042.723328
Produced by FastQC (version 0.11.5)