FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10619096
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG45200.070.951983
TACTCGG57900.069.947243
CCGAGTA47900.068.741462
CTACTCG61850.065.162242
GAGTAGC119450.064.0465554
GCTGGGA462550.062.1918379
AGTAGCT203300.062.113065
GCTACTC153600.061.718991
GTAGCTG206650.061.1344346
CCCGAGT63750.057.4970741
GGAGGCT366100.055.9308979
CTCGGGA74950.055.3401875
TCGGGAG76500.055.3056686
TAGCTGG237550.054.2060437
ACTCGGG74600.054.1000184
AAAGTGC307150.053.844944
GTGCTGG314050.053.6774947
AGTGCTG317850.053.1816756
AAGTGCT322150.052.5430955
CCCAAAG379250.052.183861
Produced by FastQC (version 0.11.5)