FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962322
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT801.14308E-535.3224075
CCGAGTA10350.028.2152842
ACTCGCG1205.7539637E-627.4734483
CTCGCGT1057.322484E-526.9123084
CGAGTAG10800.026.6012743
AGTAGCT40450.026.5463815
GAGTAGC25000.026.3740624
GCTGGGA71200.026.2682259
GTAGCTG41550.025.9043546
TACTCGG8950.025.7851353
CCCGAGT13950.025.3254341
TTGGGAG57750.025.2300436
GGAGGCT55650.025.0795049
TAGCTGG46600.023.7987297
GGGAGGC69150.023.7170628
CTACTCG8950.023.6822282
GCTACTC23100.022.2271791
CGGAGGT10850.021.6554857
CCCAAAG49950.021.3129121
TGGGAGG73300.021.15627
Produced by FastQC (version 0.11.5)