FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962322
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN84600.2855867795600884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1003.3194738E-837.613545
CTCGCGT1309.16225E-932.5518384
CCGAGTA11900.028.4496332
AGTAGCT40500.027.9779725
TACTCGG10850.027.7358443
GCTGGGA74850.027.2929239
CGAGTAG11250.027.1676453
GAGTAGC25200.027.054774
GTAGCTG41650.026.961836
CCCGAGT14200.026.84051
TTGGGAG56450.026.3852846
CTACTCG10850.026.0023542
GGAGGCT54500.025.5924919
CGCGTTG1501.1666343E-625.0590846
GGGAGGC73950.024.0687588
GCTACTC22750.023.9922241
TAGCTGG49200.023.7717197
CCCAAAG53550.023.7245621
CTCGGGA12900.023.69075
CGCGAGT1000.001747674923.5268571
Produced by FastQC (version 0.11.5)