FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3596413
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12800.041.220343
CCGAGTA14650.037.3059732
TCGCGTT1353.3833203E-1034.895535
AGTAGCT49600.034.476925
GTAGCTG49850.034.224366
GAGTAGC32050.033.8067444
GCTGGGA92100.033.5217869
CCCGAGT19400.032.7916071
CTCGCGT1755.456968E-1232.3032884
TACTCGG11000.030.4066933
TAGCTGG59050.030.3198097
TTGGGAG66450.030.059926
GCTACTC27400.028.7208041
GGAGGCT65550.028.6753489
CTACTCG10850.028.6597392
CCCAAAG63650.026.2821331
CGCACTA909.530645E-426.10687894
GGGAGGC87400.026.0766458
CGGAGGT13400.025.9506557
AAAGTGC51200.025.5786934
Produced by FastQC (version 0.11.5)