FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8286904
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG47850.079.9130553
CCGAGTA51550.075.9647452
GAGTAGC117950.069.485244
AGTAGCT187850.068.653675
TACTCGG56650.068.1823353
CTACTCG56750.066.6168062
GTAGCTG192950.066.4386
GCTACTC136400.065.280321
CCCGAGT63650.065.128181
GCTGGGA428200.064.905799
TAGCTGG218400.060.261237
GGAGGCT333150.058.2805679
AAAGTGC267550.056.6379974
AAGTGCT274600.055.9353265
TTGGGAG269050.055.747036
CCAAAGT294600.054.5716862
AGTGCTG275700.054.5193256
GTGCTGG278650.054.4747167
CCCAAAG323450.054.359171
TCGGGAG75150.053.9717676
Produced by FastQC (version 0.11.5)