FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10436310
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG47350.074.377743
CCGAGTA50450.071.116762
GAGTAGC121600.065.82494
TACTCGG55750.064.472333
GCTGGGA439800.064.092259
GCTACTC140400.063.5284651
AGTAGCT205900.063.2108655
CCCGAGT60550.063.1312751
CTACTCG57600.062.74692
GTAGCTG211950.060.9990436
TAGCTGG226050.059.5502787
GGAGGCT334950.057.087029
TCGGGAG70750.055.790186
CGGAGGT75900.054.25737
CTCGGGA71700.052.2312135
GTGCTGG311150.051.924297
AAAGTGC316850.051.2733964
AAGTGCT322800.051.2061045
AGTGCTG321100.050.7711266
TTGGGAG269850.050.7679336
Produced by FastQC (version 0.11.5)