FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10858697
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG64950.072.996423
CGAGTAG50400.071.109943
CTACTCG64550.070.8900762
CCGAGTA54350.067.890692
GAGTAGC131150.067.397644
AGTAGCT216450.066.126245
GCTGGGA501950.065.838019
GCTACTC161000.065.820861
GTAGCTG226100.064.113216
CCCGAGT66700.061.12981
TAGCTGG254300.059.1835257
TCGGGAG85900.058.764046
CTCGGGA83950.058.0390555
GGAGGCT392750.057.413449
AAAGTGC343000.056.12794
AAGTGCT351400.055.443665
GTGCTGG347000.055.234677
AGTGCTG347300.054.9799736
CCCAAAG410550.054.751971
ACTCGGG87300.054.2983254
Produced by FastQC (version 0.11.5)