FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10858697
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG71350.074.769873
CGAGTAG52150.074.0296253
CCGAGTA53900.073.588432
CTACTCG73150.072.29742
GAGTAGC130300.069.2521444
GCTACTC170100.068.026941
AGTAGCT213000.065.763425
GCTGGGA500150.065.6832059
CCCGAGT67750.063.6334571
GTAGCTG219150.063.484476
TAGCTGG247100.059.4529657
GGAGGCT413350.059.3153089
TCGGGAG91200.059.1360366
AAAGTGC335500.058.560034
ACTCGGG90700.058.307944
CTCGGGA89950.058.278055
CCCAAAG402750.057.281271
AAGTGCT347600.057.18565
CCAAAGT372750.056.3970642
AGTGCTG348600.055.6085476
Produced by FastQC (version 0.11.5)