FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8384910
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG44100.070.8042143
CTACTCG44100.068.645142
CGAGTAG34300.065.130013
GCTACTC107900.063.76951
CCGAGTA36850.061.656032
GAGTAGC88400.060.8084224
GCTGGGA318700.057.450159
CTCGGGA54650.056.6711775
GGAGGCT269950.056.540449
AGTAGCT148150.056.4607165
TCGGGAG58800.055.9094286
GTAGCTG147100.055.8282786
CCCGAGT45950.054.703461
TAGCTGG159450.053.493647
ACTCGGG58600.053.17614
TTGGGAG228450.052.7553376
AAAGTGC212350.049.552424
CCCAAAG260700.049.4995731
AAGTGCT217400.049.131185
CGGAGGT58800.048.5693867
Produced by FastQC (version 0.11.5)