FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3259861
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG14300.046.012693
CCGAGTA14950.045.5840072
GAGTAGC37400.042.8517384
GTAGCTG56850.042.575766
AGTAGCT57650.042.2295155
GCTGGGA102400.039.747549
TAGCTGG64450.039.2324147
CCCGAGT18550.038.589181
TTGGGAG79900.035.9401666
CGGAGGT14700.034.849367
AAAGTGC48400.034.375074
CCCAAAG62300.034.0165561
CTACTCG11050.033.6008642
AAGTGCT49400.033.298665
GCACTTT66550.032.905671
CCAAAGT57300.032.8908732
TTTGGGA76950.032.7372255
GGAGGCT75900.032.32379
TACTCGG12350.031.5861593
ACTTTGG70300.031.4215933
Produced by FastQC (version 0.11.5)