FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3259861
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT57050.042.910355
GAGTAGC35700.041.3277174
GTAGCTG59000.040.3771746
CGAGTAG15250.040.05473
TACTCGG11800.039.81973
GCTGGGA106400.039.789019
TTGGGAG85650.039.6634836
CCGAGTA15400.039.358842
TAGCTGG63500.038.699737
CTACTCG12100.038.05522
GCTACTC28800.038.02041
GCACTTT68900.036.4911731
CCCGAGT18500.036.3261
GGAGGCT85300.035.2542049
TTTGGGA81050.035.0737575
ACTTTGG74200.034.512193
AAAGTGC51300.033.797844
CACTTTG75550.033.6462022
CGGAGGT20250.033.6451877
AAGTGCT53600.033.224195
Produced by FastQC (version 0.11.5)