FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432702
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG14900.052.0454063
CCGAGTA15950.049.7978672
AGTAGCT61200.043.4660075
GAGTAGC40100.043.1308634
CCCGAGT20350.042.57131
GTAGCTG61900.042.3670546
GCTGGGA107600.038.91919
TAGCTGG68500.038.4221957
TTGGGAG81300.035.32126
AAAGTGC49800.033.691754
AAGTGCT50700.033.649875
TACTCGG10600.032.810333
CGGAGGT15400.032.349677
CTACTCG11250.030.9146212
CCCAAAG66550.030.7754731
GGAGGCT74150.030.6783229
CCAAAGT59250.030.3011952
GGGAGGC104300.030.2809628
TTTGGGA80950.030.2486235
AGTGCTG56200.030.105856
Produced by FastQC (version 0.11.5)