FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432702
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA15650.046.2326132
CGAGTAG16300.045.5419433
AGTAGCT58750.044.544675
TACTCGG12450.042.2659653
CTACTCG11800.042.2051962
GTAGCTG61950.042.1678926
GCTGGGA109850.041.402839
GCTACTC31900.041.3948481
GAGTAGC38100.041.1879234
TTGGGAG89150.040.3697286
TAGCTGG67800.038.8067057
AAAGTGC54850.037.946834
CCCGAGT20200.037.45461
GCACTTT72350.037.0226061
CCCAAAG69650.035.6915361
GGAGGCT86500.035.414929
TTTGGGA85950.035.3130385
AAGTGCT58700.035.2979055
CCAAAGT62350.035.190262
ACTTTGG76250.034.444043
Produced by FastQC (version 0.11.5)