FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5437793
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2050.052.6829228
TCGGCGG3500.032.9142889
TATCGGC3850.026.1818187
TTATCGG5151.8189894E-1220.9708736
TCGTCGC2150.014.06511615-19
CGATCGG1455.4169504E-913.903449130-134
ATTATCG9902.8267095E-911.6363645
CCGATCG1358.567503E-510.66666755-59
GCGATCG1902.3327266E-710.610526130-134
CTCGTCG2858.185452E-1110.10526415-19
CGTCGCT2901.196895E-99.43448315-19
CCGGCGA3207.403287E-109.030-34
CGATCGC1900.00259777117.578947560-64
CGGCGAG4201.5235855E-87.230-34
TCCGGCG3102.213547E-56.967742425-29
GGTTCGT5302.9765943E-86.24905715-19
GTTACCG4001.8038538E-56.1220-24
CAGTACC32257.7398E-95.80465138
CGTTACC4252.8111736E-45.421176415-19
GCGGGTT5551.6576283E-55.18918910-14
Produced by FastQC (version 0.11.5)