FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5299429
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1750.090.514298
TATCGGC4150.039.9036147
TCGGCGG4250.037.2705889
TTATCGG5300.028.5283016
ATTATCG9150.018.8852465
TCGTCGC1354.7699723E-712.815-19
CGATCGG1252.8149589E-612.672130-134
TCCGGCG3450.010.0173925-29
CCGGCGA3700.09.7297330-34
CGGCGAG3905.456968E-128.86153930-34
GCGATCG2005.2073025E-58.64130-134
CTCGTCG1953.7442532E-48.12307715-19
GTTACCG3954.658432E-97.65569620-24
ATCCGGC5202.8903742E-96.64615425-29
TCGTTAC5151.650551E-86.43106815-19
CGTTACC5109.210089E-86.211764315-19
GGTTCGT5201.347471E-76.092307615-19
TTACCGA5402.812576E-75.86666720-24
CGTCGCT2950.00443878635.85762715-19
TACCGAA6151.664921E-85.85365920-24
Produced by FastQC (version 0.11.5)