FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044367
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGC1206.73208E-915.615-19
CTCGTCG1405.121001E-813.371428515-19
CGTCGCT1851.8964438E-610.11891915-19
CAGTACC17209.375159E-56.279078
GACCTCC20704.579353E-66.26086952
AGACCTC23054.1207622E-65.93492361
CCAGTAC17658.818172E-45.7110487
TACAGCG3400.00351246965.505882380-84
CGCTGGT5551.6572927E-55.18918920-24