FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459681
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG808.547431E-963.08
TATCGGC1502.7657734E-838.47
TCGGCGG2303.316947E-521.9130449
TTATCGG2557.34322E-519.7647066
ATTATCG5107.547532E-512.7058835
CTCGTCG1501.2612327E-712.480000515-19
TCGTCGC1356.649834E-611.73333415-19
CGTCGCT1602.9186594E-711.715-19
CCGATCG1150.00291506210.01739155-59
CCAGTAC13001.8324408E-68.3076937
GGCGTCG1700.0081574217.6235294125-129
CAGTACC14206.4402866E-67.60563338
AGTACCT17401.5477024E-56.620699
TCGTTAC2400.00339859446.615-19
CCGTAAA4353.9111736E-45.296551735-39
GACCTCC19350.00281335275.20930242