FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620557
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG851.8007995E-1067.764718
TATCGGC1452.0505468E-839.724147
TCGGCGG1604.881076E-836.09
TTATCGG2156.510345E-726.7906996
ATTATCG3202.0474767E-518.05
TCGTCGC1053.9458246E-715.08571415-19
CCGATCG900.005438315711.255-59
TCCGGCG1050.001367688810.97142825-29
CTCGTCG1502.1396047E-510.5615-19
CCGTAAA2201.7118587E-59.8181828
CGTCGCT1502.482636E-49.615-19
CCAGTAC10559.787509E-79.55450257
GACCTCC13855.385482E-88.8375442
CAGTACC9954.650423E-47.95979938
AGTACCT11450.00207268546.9170319
TCCAGTA13852.9810538E-46.75812246
AGACCTC14555.4340076E-46.432991
ACCGTAA2550.00639559656.21176535-39
CTCCAGT19103.9146948E-45.65445045
ACCTCCA24306.0073333E-55.33333353
Produced by FastQC (version 0.11.5)